Seed sowing part 1

Today sowed seeds for Trias nasuta. This seed was gathered in Mar. 2011 and stored in folded wax paper. I apologize for lack of photos. However I have been doing this solo so it's difficult to photograph and do what needs done. I will try to work out a way to get some photos, even if that means just videoing the process.

Now for the process: Seeds were laid out onto a piece of copy paper that had been folded to create a crease. The seeds were then funneled into the syringe with the plunger removed. Seeds were disinfected 2 different ways. First  the majority of the seeds were treated in a syringe filled 1part bleach to 9 parts water for ten minutes. A small hole was drilled in the rubber cap and a small piece of coffee filter was inserted. This was then shaken for 10 minutes. After 10 minutes the solution was expelled out and pressure cooked distilled water was drawn up. This was shaken slightly then allowed to rest for 2 minutes. The distilled rinse was repeated 3 times whereupon on the 3rd expulsion 2 ml of seed water mixed was left in the syringe. The cap was then removed and 1ml of seed + water was dispensed into 1 poly container of cornstarch media, 1 poly container of p723. Then one baby food jar (pre-sterilized with 50% bleach solution) had 1 sterilized coffee filter soaked in OSP1 baby food media placed in the bottom. 1ml of water was then drawn into the syringe gentle swirled then dispensed into the baby food jar. The plunger was then removed and dabbed onto the soaked coffee filter to remove any additional seed not dispensed. Cap then screwed on tight and everything was sprayed with bleach water prior to removing from the glove box.

Seed that failed to fall into the syringe or still left on the wax paper was brushed into 1 soda bottle with p658 (in open air not in glove box) then medical grade cotton balls were soaked in 100% bleach and placed on the top of the bottle opening and cap then place on top. Cotton removed in glove box and cap screwed tight.