Wednesday 15 February 2012

Orchid Media Preparation cont'd

A little delayed but on 2/12/1012 flasks were made using a modified OSP1 media:
2 level tsp sucrose
1/2 level tsp plant pro 20-20-20
138ml banana baby food (no added sugar version)
mixed with enougth distilled water to equal 1L then pour into two mason jars and pressure cooked. Also pressure cooked 10 coffee filters (to be used in sterile jars for seed flasking) and also a 1 qt container of perlite was pressure cooked for replating later.

Flasks were also made using a modified cornstarch method by CD Moulton:
1/4 cup of cornstarch
1 tbsp + 1 tsp sugar
1/2 tsp plant pro 20-20-20
1 L humidifer water
This was dispensed into 3 mason jars which was pressure cooked as well as 1 polypro container that was pressure cooked as well. Also 3 polypropolyene containers were cooked in the microwave for 4:30 mins. then place in a bag sprayed with bleach to cool.

20 Flasks  were produced of P658 as well:
5 vented polypropylene
5 unvented polypropylene
1 unvented mason jar
2 vented mason jars
1 tall recycled food jar with metal lid - vented
3 vented plastic soda bottles
3 unvented soda bottles
- I experienced more melting this time with the soda bottles when dispensing the boiling media. I found that the bottoms are more reistant to the boiling so the bottles standing up have less deformitiy. Next time will have to try to fill standing then rotate to the side as it cools.

Also I have noticed that all of the polypropolene containers have a large amount of condesation in them where the mason, and food jars have nearly none. Perhaps I do not vent the poly containers enough. Time will tell if this becomes a problem in the future.








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